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Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
One Shot PIR1 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).
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Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.
BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.
One Shot MAX Efficiency DH10B T1 Phage-Resistant E. coli have all the benefits of the DH10B genotype, with the addition of the tonA (also known as fhuA) genotype for resistance to T1 and T5 phage infection.
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Useful host for transformation of pGEM Vectors and production of ssDNA from M18 or phagemid vectors. JM109 is recA- and lacks the E. coli K restriction system. Can be used for blue/white screening.
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Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a ribosomal binding site. No blue/white selection.
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